精準(zhǔn)定量DNA Marker VI

● 產(chǎn)品編號(hào)及規(guī)格：

    RTM406-02       100T (2×250μl) 

● 產(chǎn)品簡(jiǎn)介：

本產(chǎn)品是由6條帶狀雙鏈DNA條帶組成，適用于瓊脂糖凝膠電泳中DNA條帶的分析。產(chǎn)品中已含有1×loading buffer，可根據(jù)實(shí)驗(yàn)需要，直接取2-5μl電泳，使用方便，電泳圖像清晰。6條帶的大小分別為250，1000，2500，5000，7000，10000bp。其中2500bp條帶含量為100ng/5μl，顯示加亮帶，其余條帶濃度為50ng/5μl。

● 儲(chǔ)存條件：

  4℃ 貯存6個(gè)月（-20℃貯存一年）。

● 使用方法：

1. 取2-5μl本產(chǎn)品加入到瓊脂糖凝膠的加樣孔中就行電泳。

注：根據(jù)電泳梳子的厚度和寬度確定上樣量。每1mm×1mm（厚度×寬度）加樣孔上樣1μl；窄齒梳子（一般為1mm×2mm）上樣2μl；寬齒梳子（一般為1mm×5mm）上樣5μl。如果使用厚齒梳子或?qū)捰?/span>5mm的梳子，可以適當(dāng)調(diào)整上樣量。

2. 建議電泳條件：凝膠濃度為1.0％，凝膠長(zhǎng)度5-7cm，電泳電壓4-10v/cm，電泳時(shí)間25-30分鐘。

3. 通過EB染色后紫外燈下觀察條帶。

注：如果使用Goldview等染料就行染色，由于靈敏度比EB低，請(qǐng)酌情增加Marker的上樣量。

● 注意事項(xiàng)：

1. 瓊脂糖的質(zhì)量對(duì)DNA的電泳有很大影響，電泳時(shí)請(qǐng)盡量使用質(zhì)量?jī)?yōu)等的瓊脂糖。

2. 請(qǐng)使用新鮮配制的電泳緩沖液和新鮮配制的瓊脂糖凝膠進(jìn)行電泳，以保證Marker良好的分離效果。

使用DNA Marker產(chǎn)品發(fā)表部分文章列表：

1. [2021 IF=1.17] Dendrostoma covidicola sp. nov. (Erythrogloeaceae, Diaporthales) on Fagus sylvatica from Sichuan Province, China.

Author: MILAN C. SAMARAKOON, ITTHAYAKORN PROMPUTTHA & JIAN-KUI (JACK) LI

Marker: RTM415，D2000 DNA ladder

Journal: Phytotaxa. 2021，483 (2): 085–094.

Institution：University of Electronic Science and Technology of China

Paper link：https://doi.org/10.11646/phytotaxa.483.2.1

2.[2021 IF=6.057] MUC1 detection and in situ imaging method based on aptamer conformational switch and hybridization chain reaction.

Marker :RTM403，DNA Marker III

Author: ZihanSong，ShengCai.

Journal: Talanta. Vol 239, 2022, 123129

Institution： Zhejiang University

Paper link：https://doi.org/10.1016/j.ijbiomac.2021.11.194

3. [2021 IF=3.24] Curing of Plasmid pXO1 from Bacillus anthracis Using Plasmid Incompatibility.

Marker :RTM404，DNA Marker IV

Author: Xiankai Liu, Dongshu Wang, Huagui Wang, Erling Feng, Li Zhu, Hengliang Wang

Journal: PLos one，January 2012 | Volume 7 | Issue 1 | e29875

Institution：State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology

Paper link： https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0029875

4. [2021 IF=3.24] Identification of a Novel Fungus, Leptosphaerulina chartarum SJTU59 and Characterization of Its Xylanolytic Enzymes.

Marker :RTM409，100bp DNA ladder

Author: Qiong Wu, Yaqian Li, Yingying Li, Shigang Gao, Meng Wang, Tailong Zhang, Jie Chen

Journal: PLos one，September 2013 | Volume 8 | Issue 9 | e737

Institution：School of Agriculture and Biology, Shanghai Jiao Tong University

Paper link：https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0073729

5. [2021 IF=3.08] Low genetic diversity and local adaptive divergence of Dracaena cambodiana (Liliaceae) populations associated with historical population bottlenecks and natural selection: an endangered long-lived tree endemic to Hainan Island, China.

Marker :RTM409，100bp DNA ladder

Author: D.-J. Zheng, L.-S. Xie, J.-H. Zhu，Z.-L. Zhang

Journal: Plant Biology，14 (2012) 828–838

Institution：Key Laboratory of Crop Genetics and Breeding of Hainan Province, Hainan Academy of Agricultural Sciences

Paper link：https://onlinelibrary.wiley.com/doi/10.1111/j.1438-8677.2011.00559.x

6. [2021 IF=3.24] Envelope Proteins ofWhite Spot Syndrome Virus (WSSV) Interact with Litopenaeus vannamei Peritrophin-Like Protein (LvPT).

Marker :RTM415，D2000 DNA ladder

Author: Shijun Xie, Xiaojun Zhang, Jiquan Zhang, Fuhua Li, Jianhai Xiang

Journal: Plos One，Published: December 21, 2015

Institution：Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences

Paper link： https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0144922

7. [2021 IF=1.17] Rhopalostroma brevistipitatum sp. nov. from Thailand with an extended generic description for Rhopalostroma.

Marker :RTM415，D2000 DNA ladder

Author: DINUSHANI A. DARANAGAMA, XINGZHONG LIU, SUNITA CHAMYUANG, MARC STADLER，ALI H.BAHKALI, KEVIN D. HYDE

Journal: Phytotaxa，2015，227 (3): 229–242

Institution：State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences

Paper link：https://www.biotaxa.org/Phytotaxa/article/view/phytotaxa.227.3.2/0

8. [2021 IF=20.37] A multi-locus phylogenetic evaluation of Diaporthe (Phomopsis)

Marker :RTM415，D2000 DNA ladder

Author: Dhanushka Udayanga & Xingzhong Liu &Pedro W. Crous & Eric H. C. McKenzie &Ekachai Chukeatirote & Kevin D. Hyde

Journal: Fungal Diversity (2012) 56:157–171

Institution：State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences

Paper link：https://link.springer.com/article/10.1007/s13225-012-0190-9

9. [2021 IF=2.9] An easily-performed high-throughput method for plant genomic DNA extraction.

Marker :RTM421，λ DNA/HindIII

Author: Deshui Yu, Ju Zhang, Guangxuan Tan, Ningshu Yu, Qiuyue Wang, Qiqi Duan, Xin

Qi, Mingjiao Cheng, Chunxue Yan, Zhangkun Wei, Zhenmiao Yu, Wenchao Huang, Chengwei Li

Journal: Analytical Biochemistry Volume 569, 15 March 2019, Pages 28-30

Institution：Key Laboratory of Plant Genetics and Molecular Breeding, Zhoukou Normal

University

Paper link：

https://www.sciencedirect.com/science/article/abs/pii/S0003269718310637?via%3Dihub

10. [2021 IF=2.88] Genetic diversity and genetic structure of Decalobanthus boisianus in Hainan Island, China.

Marker :RTM415，D2000 DNA ladder

Author: Huan Jiang，Wenxing Long， Hui Zhang，Chengneng Mi，Tao Zhou，Zongzhu Chen

Journal: Ecology and Evolotion Volume 569, 15 March 2019, Pages 28-30

Institution：Institute of Tropical Agriculture and Forestry, Hainan University

Paper link： https://doi.org/10.1002/ece3.5127

11. [2021 IF=5.64] Identi?cation of a Novel Hypovirulence-Inducing Hypovirus From Alternaria alternata.

Marker :RTM406，DNA Marker VI

Author: Huan Li, Ruiling Bian, Qian Liu, Liu Yang, Tianxing Pang, Lakha Salaipeth,

Ida Bagus Andika, Hideki Kondo and Liying Sun

Journal: Frontiers in Microbiology May 2019 | Volume 10 | Article 1076

Institution：State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University

Paper link： https://www.frontiersin.org/articles/10.3389/fmicb.2019.01076/full

12. [2021 IF=2.6] Natural occurrence of Alternaria mycotoxins in wheat and potential of reducing associated risks using magnolol.

Marker :RTM415，D2000 DNA ladder

Author: Dongmei Jiang, Dizhe Wei, Hongtao Li, Liuqing Wang, Nan Jiang,Yunchao Li and Meng Wang

Journal: Journal of the Science of Food and Agriculture Volume101, Issue7，2021，3071-3077

Institution：Beijing Research Center for Agricultural Standards and Testing, Beijing Academy of Agriculture and Forestry Sciences

Paper link：https://onlinelibrary.wiley.com/doi/10.1002/jsfa.10901

13. [2021 IF=20.37] Taxonomy, Phylogeny, Molecular Dating and Ancestral State Reconstruction of Xylariomycetidae (Sordariomycetes)

Marker :RTM415，D2000 DNA ladder

Author: Kevin D Hyde，Jian-Kui Liu

Journal: Fungal Diversity volume 112, pages 1–88 (2022)

Institution：School of Life Science and Technology, Center for Informational Biology, University of Electronic Science and Technology of China

Paper link：https://link.springer.com/article/10.1007/s13225-021-00495-5